Researchers From Tianjin Medical University Finds That Quercetin Is One Of The Many Phytochemicals In TCM Herbs That May Inhibit SARS-CoV-2 Virus.

Researchers From Tianjin Medical University Finds That Quercetin Is One Of The Many Phytochemicals In TCM Herbs That May Inhibit SARS-CoV-2 Virus.


COVID-19 Drug News: A research conducted by scientists from Tianjin Medical University that has been published in the peer reviewed Computational and Structural Biotechnology Journal indicates that Quercetin is one of the many phytochemicals in TCM (Traditional Chinese Medicine) herbs that may inhibit SARS-CoV-2 Virus.

Utilizing computational methods, the study team identified three phytochemicals in traditional Chinese medicine that could be used against SARS-CoV-2: Quercetin, Puerarin and Kaempferol.

Importantly of the three compounds, Quercetin showed the highest binding affinity to both the ACE2 receptor and the receptor-binding domain of the SARS-CoV-2 spike protein, and could thus provide a dual synergistic effect.

The SARS-CoV-2 coronavirus infects human hosts by binding with the human angiotensin-converting enzyme 2 (ACE2) receptor on their cells, notably the epithelium lining the respiratory tract. The receptor-binding domain (RBD) of the coronavirus spike protein binds to ACE2 followed by membrane fusion to the host cell, thus allowing the virus to infiltrate the cell and commence replication.

TCM or Traditional Chinese Medicine, widely used for many diseases, showed therapeutic effects during the 2003 SARS-CoV epidemic.

The receptor-binding domain or RBD of the SARS-CoV-2 has significant structural homology with SARS-CoV. Although the use of Chinese herbs with modern medicine has shown benefits in COVID-19 patients, several components are present in the herbs and have complex interactions, making it challenging to uncover the molecular mechanisms responsible for its therapeutic effects.

Numerous computational studies have helped predict active compounds in the medicinal herbs with the potential to accelerate traditional medicine-based drug discovery.

The study team used computational analysis to discover potential molecule candidates against SARS-CoV-2 infection. Utilizing the Traditional Chinese Medicine Pharmacology database, they screened for molecules that could target ACE2.

The team identified the compound puerarin that could target ACE2. Then, they screened for Chinese herbs that have this compound in the database and found five. Furthermore, since it is thought that compounds in the same herbal medicine have synergistic properties, they expanded their search to include all the compounds in the five herbs to arrive at 41 compounds.

However upon analyzing which compounds were present in the maximum number of herbs, they found puerarin was present in all the five herbs, and quercetin and kaempferol were present in three herbs.

Subsequently, they predicted potential drug targets of the selected compounds using the database, leading to 240 possible targets. Upon further analysis, they selected puerarin, quercetin, and kaempferol for further study.

The study team next performed molecular docking analysis to determine potential binding sites and binding affinity to ACE2. All the three compounds could bind on the same region of ACE2, which is located some distance from the binding position of SARS-CoV-2. It is likely the compounds are causing changes in conformations rather than competing with the spike protein to bind to ACE2.

Interestingly, it was found that Quercetin had the highest binding affinity, forming both strong and weak hydrogen bonds.


Quercetin could bind to RBD domain of S-protein with a high binding affinity. (A) Hydrophilic-hydrophobic interaction between (i) quercetin and SARS-CoV-2 Spike in candidate protein binding pocket, and (ii) quercetin and relative amino acids. (B) The KD of the SARS-CoV-2 Spike RBD protein with a series of concentrations of quercetin was calculated by SPR.

The team also experimentally determined the binding of the three compounds to ACE2 using surface plasmon resonance. Similar to the theoretical analysis, they found quercetin had higher binding affinity to ACE2 than puerarin. They also observed that puerarin affected the binding of spike protein to ACE2, and quercetin almost completely disrupted the spike protein binding to ACE2.

Significantly molecular docking analysis showed that quercetin has high binding affinity to the spike protein.

Using pathway enrichment analysis for the COVID-19-related genes, the study team found quercetin affected the immune-modulation and viral infection activities.

Quercetin has been found in all the 26 Chinese herbal medicines advised by traditional Chinese medicine practitioners to combat COVID-19.

Quercetin showed a higher binding affinity to both ACE2 and the RBD of the spike protein. The dual binding effect of quercetin could therefore be synergistic and provide a strong antiviral effect against SARS-CoV-2.

Furthermore, since analysis suggested that quercetin could affect immunomodulation, and because studies have shown patients with severe COVID-19 disease tend to experience cytokine storms, quercetin could help alleviate symptoms in such cases. will be providing more COVID-19 research updates on a constant basis.

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